• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    Formulation of coating for the control of post-harvest citrus rot, comprising, at least, fungal chitosan, hydroxytyrosol and peppermint. (Machine-translation by Google Translate, not legally binding)
    公开号:ES2660010A1
    申请号:ES201631223
    申请日:2016-09-19
    公开日:2018-03-20
    发明作者:Roberto VILAPLANA PÉREZ
    申请人:Citromil S L；Citromil SL；
    IPC主号:
    专利说明:

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    FORMULATION OF COATING FOR THE CONTROL OF POWER IN
    POST-HARVEST OF CITRUS
    DESCRIPTION
    Technical sector
    The present invention fits within the industry
    Agrifood and refers to the application as a coating of a formulation based on fungal chitosan, hydroxytyrosol and peppermint as active materials with fungal ability to control green and blue rot in post-harvest citrus, especially lemons.
    State of the art
    Over the years, different methods have been used to control fungal attacks in citrus distribution warehouses. Formulations made from synthetic compounds are widely used in the citrus industry for disease control, although the current trend in the food industry indicates a growing concern in terms of safety and health, as well as a greater commitment to the environment. ambient. Currently the use of imidazoles is widespread in the fight against this type of microorganisms.
    Thus, it is known document ES 2439616 which describes a coating formulation comprising at least one aldehyde or phenol of plant origin with fungistatic activity, preferably cinnamaldehyde and a fungistatic food additive for controlling post-harvest diseases in fruits and vegetables , preferably citrus.
    Chitosan-based coatings of animal origin (mainly invertebrate cytoexkeletons such as crabs) have
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    been widely studied as coatings in fruits and vegetables. In addition, its antimicrobial capacity used as a preservative in foods and beverages has been verified but taking into account its origin from crustaceans and, given the possibility of food allergies, it was decided to experiment with a similar compound but with plant origin. This type of chitosan comes from the cell wall of the Arpergillus Niger fungus.
    An example of this type of chitosan is the product with denomination
    commercial "NO BRETT INSIDE" marketed under this name by the laboratories KITOZYME and LALLEMAND for use in wine ( http://www.scottlab.com/products-129.aspx).
    Fungal activity of this type of chitosan against Brettanomyces Bruxellensis in wine has been described in the state of the art. This yeast develops at any stage of winemaking, especially in the aging phase. It is easy to use, biodegradable, non-toxic, non-allergenic and has been approved by the OIV (International Wine Organization) in July 2009 and by the European Union in December 2012 as a new practice in the wine code.
    However, in the state of the art no formulation of fungal chitosan to fight against citrus rot and, especially, rot in lemons is described.
    Description of the invention
    An object of the present invention is a coating formulation for the control of post-harvest citrus rot comprising at least fungal chitosan, hydroxytyrosol and peppermint. In a particular embodiment, the fungal chitosan is a chitosan that comes from the cell wall of the Arpergillus Niger fungus.
    In a second object of the invention a method of obtaining the indicated formulation comprising a first step of diluting
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    fungal chitosan in water by continuous stirring and addition of lactic acid; mix hydroxytyrosol, peppermint and an emulsifier in continuous stirring; and once the emulsion has been formed, it is incorporated into the fungal chitosan mixture until the solution is completely dissolved with the hydroxytyrosol emulsion.
    It is another object of the invention to use the indicated formulation to treat at least one citrus product. In a particular embodiment, said citrus product is lemon.
    Finally, it is another object of the invention to apply the coating formulation on the post-harvest product by a technique selected from immersion, drencher type shower or in-line spray.
    Throughout the description and the claims the word "comprises" and its variants are not intended to exclude other technical characteristics, additives, components or steps. For those skilled in the art, other objects, advantages and features of the invention will be derived partly from the description and partly from the practice of the invention. The following examples and drawings are provided by way of illustration, and are not intended to restrict the present invention. In addition, the present invention covers all possible combinations of particular and preferred embodiments indicated herein.
    Statement of a detailed embodiment of the invention
    The object of the present invention is based on the application by dipping or drencher-type showers or in-line spray of a formulation based on natural compounds with antifungal capacity on citrus fruits after their collection to control the development of P.Digitatum and P fungi .Itallicum responsible for green and blue rot respectively.
    The active substances responsible for fungicidal capacity are chitosan of fungal origin, for example, the product marketed as NO BRETT INSIDE® by the firm LALLEMAND®. The second principle
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    Active is hydroxytyrosol from the olive and peppermint essential oil (peppermint peppermint). The synergy of these compounds has demonstrated a rot control similar to that carried out with other types of fungicides of habitual use and synthetic origin based on imazalil (see, for example, document ES2439616).
    The development of resistance and, above all, the accumulation of toxic residues in the citrus bark makes the application of easily biodegradable natural products necessary.
    The novelty of this formulation is the usefulness of this type of product (fungal chitosan) to fight against the rot of citrus and, preferably, lemon, a utility that to date is unknown in the state of the art.
    The formulation includes other ingredients, such as hydroxytyrosol, which has also not been described for use in formulations such as that recommended in the present invention.
    Hydroxytyrosol is a natural product that comes from the olive tree ( https://es.wikipedia.org/wiki/Hydroxytyrosol) and which has been used as a microbicidal gel against the AIDS virus (WO2011067302) with antioxidant and microbial characteristics demonstrated in the state of the art.
    Finally, the last active component of the formulation is peppermint whose antimicrobial activity is widely described in the state of the art.
    Example
    For the development of the formula, tests with lemons were carried out to prove its effectiveness. Lemons were inoculated with the fungi responsible for the rot (P.Digitatum and P.Itallicum fungi responsible for the green and blue rot respectively) and
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    They performed controls without inoculating to compare the treatments. The different treatments evaluated were compared with a classic synthetic fungicide currently used (imazalil) and, thus, compare the effectiveness of the new formulation.
    The form of application of this type of coating was by immersion for three minutes.
    The process for preparing the formulation object of the invention was the following:
    First, a solution of chitosan in water was performed by continuous stirring and addition of lactic acid. Lactic acid is necessary to facilitate the dissolution of fungal chitosan in water.
    Secondly, the hydroxytyrosol, peppermint (peppermint) and Tween 80 (emulsifier) were mixed and led to continuous agitation. Once the emulsion formed, it was incorporated into the chitosan mixture and kept under stirring until the mixture was completely dissolved.
    Citrus fruits, in this example lemons, were then immersed in this mixture for three minutes and left in a chamber at a constant temperature and humidity until rot.
    权利要求:
    Claims (1)
    [1]
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    1 - Coating formulation for the control of post-harvest citrus rot that is characterized in that it comprises at least fungal chitosan, hydroxytyrosol hydroxytyrosol and peppermint.
    2 - The formulation of claim 1 wherein the fungal chitosan is a chitosan that comes from the cell wall of the Arpergillus Niger fungus.
    3 - A process for obtaining the formulation of any of claims 1-2 characterized in that it comprises a first step of diluting fungal chitosan in water by continuous agitation and addition of lactic acid; mix hydroxytyrosol, peppermint and an emulsifier in continuous stirring; and once the emulsion has been formed, it is incorporated into the fungal chitosan mixture until the solution is completely dissolved with the hydroxytyrosol emulsion.
    4 - The use of the formulation of any of claims 12 to treat at least one citrus product.
    5 - The use of the formulation according to claim 4 wherein the citrus product is lemon.
    6 - The use of the formulation according to any of claims 4-5 for the control of the development of fungi P. Digitatum and P.Itallicum.
    7- The use of the formulation according to any of claims 4-6 by means of a technique selected from immersion, drencher shower or in-line spray.
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    同族专利:
    公开号 | 公开日
    ES2660010B1|2018-10-05|
    ES2660010B8|2020-03-06|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    法律状态:
    2018-10-05| FG2A| Definitive protection|Ref document number: 2660010 Country of ref document: ES Kind code of ref document: B1 Effective date: 20181005 |
    优先权:
    申请号 | 申请日 | 专利标题
    ES201631223A|ES2660010B8|2016-09-19|2016-09-19|FORMULATION OF COATING FOR THE CONTROL OF POST-HARVEST ROT OF CITRUS|ES201631223A| ES2660010B8|2016-09-19|2016-09-19|FORMULATION OF COATING FOR THE CONTROL OF POST-HARVEST ROT OF CITRUS|
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